RT² qPCR Primer Assays use SYBR ® Green-based quantitative real-time PCR technology to provide a sensitive and reliable tool for gene expression analysis.. Each assay utilizes a proprietary and experimentally verified algorithm for the design of gene-specific qPCR primers with uniform PCR efficiency and amplification conditions. 140,00 €

2011 Jun;77(11):3551-7. doi: 10.1128/AEM.00331-11.

128,33 € eCollection 2020. 2020 Aug 19;16(8):e1008967. 128,33 € 2020 Aug 12;15(8):e0236338. Human SCLY qPCR Primer Pair 2020 Jul 31;15(7):e0233582.
doi: 10.1371/journal.pgen.1008967. 140,00 € You can customize the potential PCR amplicon's size range, Tm (melting temperature) for the primers and probes, as well as the organism. MYT1 Negative Primer Set 5 A primer hybridizes with a complementary sequence on a single-stranded target nucleotide string, and a polymerase enzyme gathers nucleotides from the surrounding mixture and extends the complementary chain. 2006 Nov 9;7:496. doi: 10.1186/1471-2105-7-496.Spandidos A, Wang X, Wang H, Dragnev S, Thurber T, Seed B.BMC Genomics. 140,00 € PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). doi: 10.7554/eLife.58057.Dwivedi N, Mondal S, P K S, T S, Sachdeva K, Bathula C, K V, K S N, Damodar S, Dhar SK, Das M.PLoS One. IL-8 Positive Primer Set 8 140,00 € A qPCR reaction is carried out much like a traditional PCR reaction. Silverchair Information Systems

Specific primers for PCR should be designed with the aid of primer design software to eliminate the complications introduced with primer-dimers and secondary structures. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. Guidelines for the design of QPCR primers are described below.


Human PLIN2 qPCR Primer Pair There are several ways to search for primers for the gene(s) of interest, such as by: GenBank accession number, NCBI protein accession number, NCBI gene ID, PrimerBank ID, NCBI gene symbol or gene description (keyword). Human SYT12 qPCR Primer Pair

The full cDNA and amplicon sequences as well as the highlighted location of primers on the amplicon are seen here and can be viewed from PrimerBank by clicking on the cDNA and amplicon sequence link found on the primer information web page (seen in Figure 1).Experimental validation data for beta-actin primers.

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